Regulatory

Part:BBa_K270000:Design

Designed by: Ann Lesnefsky   Group: iGEM09_Michigan   (2009-09-20)


Pu Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 295
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Pu promoter is not annotated on the NCBI sequence and transcribes the XylU, XylW, XylM, XylA, XylB and XylN genes. A 400 bp region between the XylU gene and the tnpA transposase was selected for amplification by colony PCR where the promoter was believed to reside.


Source

NCBI: Pseudomonas putida plasmid pWW0


References